客户评价
"In our experience this column is highly suitable for the separation of phosphates, since the
metal-free design vastly reduces tailing and enabled us to obtain narrow, intensive peaks and
quantify lipids at concentrations that were not accessible with other columns."
"参考中文:根据我们的经验,ACQUITY
Premier色谱柱非常适合分离磷酸盐,因为无金属设计大大减少了拖尾,使我们能够获得狭窄、密集的峰,并在其他色谱柱无法达到的浓度下定量脂质。"
评价者: Lorenz Waltl 因斯布鲁克大学,奥地利
"This column (Xselect eXtended Perfomance (XP)HSS) has provided amazing and very reproducible
results when coupling HPLC to MS. Great peak shapes and no retention time drifts after long
batches of analysis."
"参考中文:在做LCMS分析时,该色谱柱提供了惊人的可重复的结果。在长序列分析后,峰形状仍然非常好,且无保留时间漂移。"
评价者:Javier Jimenez Villarin 巴塞罗那大学,西班牙
"BEH columns (ACQUITY UPLC) are useable for all pH range reversed-phase separation and helped us
to replace other laborious desalting cleaning steps before LC-MS/MS experiments for peptides. We
use it for iTRAQ, phosphopeptide analysis and global proteomic analysis. Peaks are evenly
distributed across the gradient unlike strong cation exchange methods. Basic RPLC using BEH
column is our favorite offline method. Peptide separation is very different in low and high pH
condition offering a scope for better fractionation. The only one pre-step needed is 30 kDa
buffer exchange of protein lysate in urea."
"参考中文:ACQUITY UPLC
BEH色谱柱可用于所有pH范围的反相分离,并帮助我们在肽的LC-MS/MS分析之前,替代其他费力的脱盐清洗步骤。我们将其用于iTRAQ、磷酸肽分析和蛋白质组学分析。与强阳离子交换法不同,色谱峰均匀分布在梯度上。使用反相的BEH柱是我们喜欢
的方法。在低pH和高pH条件下,肽分离非常不同,为更好的分离提供了空间。"
评价者:Raghothama Chaerkady 约翰霍普金斯大学,美国
"The column (ACQUITY UPLC BEH) very well-kept the reproducibility and the separation robustness
even after ten thousand injections. Peak shapes are well-maintained at concentrations very close
to lowest limit of quantification, no or very minimum peak smoothing is needed. After-sales
cares are very professional and eager to help solving problems. When the column is conjugated
with guard column and in-line filter, the life-time and separation reproducibility are ensured.
Little expensive in the beginning, but definitely worth it in the long run."
"参考中文:该ACQUITY UPLC
BEH色谱柱即使在一万次进样后,仍能很好地保持重现性和分离稳健性。在非常接近最低定量限的浓度下,峰形状保持良好,无需或非常小的峰平滑。售后服务人员非常专业,乐于帮助解决问题。当柱与保护柱和在线过滤器结合时,可确保使用寿命和分离重现性。一开始有点贵,但从长远来看相当值得。"
评价者:Yao Chen 堪萨斯大学,美国
"Water's CSH (Charged Surface Hybrid) columns offer a significant advantage over other types of
columns. It offers superior peak shape for nearly all basic compounds, increased loading
capacity (meaning more concentrated samples or higher injection volume), rapid column
equilibration after changing mobile-phase pH (allows switching between between mobile phases for
changing retention order), and it is very stable at extreme pH ranges. Overall, it is a very
good column that can be used for a variety of analysis."
"参考中文:与其他类型的色谱柱相比,Waters的CSH(充电表面混合)色谱柱具有显著优势。它为几乎所有的碱性化合物提供了优越的峰形,增加了负载能力(意味着更浓的样品或更高的进样量),改变流动相pH后快速的柱平衡(允许在流动相之间切换以改变保留顺序),并且在极端pH范围内非常稳定。总的来说,这是一个非常好的色谱柱,可以用于各种分析。"
评价者:Tom Phillips 马里兰州农业部化学家